University of Tabriz Journal of Applied Research in Plant Protection 2383-1855 14 2 2025 06 22 Lethal and sublethal effects of matrine (Rui agro®) on the tomato leaf miner, Tuta absoluta and the predatory bug Macrolophus pygmaeus Lethal and sublethal effects of matrine (Rui agro®) on the tomato leaf miner, Tuta absoluta and the predatory bug Macrolophus pygmaeus 111 125 19084 10.22034/arpp.2025.19084 FA Rizan Mohammadi Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. Orouj Valizadegan Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. Asmar Soleymanzadeh Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. Journal Article 2024 06 16 <em>Tuta absoluta </em>is a serious pest of Solanaceae plants which is resistant to many commonly used insecticides causing economic damage worldwide. Thus, the development of integrated pest management (IPM) approaches against this pest is necessary. This study evaluated the toxicity of matrine (Rui agro®) insecticide against different biological stages of <em>T. absoluta</em>. Analysis revealed egg as the most resistant biological stage with LC₅₀ value 1064.32 mg/L. However, the sublethal concentration (LC₂₅) effect of matrine on <em>T. absoluta</em> population growth parameters was estimated using age-stage, two-sex life table theory method. The sublethal concentration (LC₂₅= 838.25 mg/L) of matrine reduced different biological stages longevity and female fecundity in comparison to control treatment (distilled water). Values of population growth parameters: the intrinsic rate of increase (<em>r</em>), net reproductive rate (<em>R</em>₀), finite rate of increase (<em>λ</em>) and gross reproductive rate (<em>GRR</em>) with LC₂₅ of matrine were significantly lower than the control. The selectivity ratio (SR) value indicated that matrine are selective and safe for predator <em>Macrolophus pygmaeus</em>. Based on the results obtained in this study, combined use of <em>M. pygmaeus</em> with matrine can be recommended in <em>T. absoluta</em> integrated management programs, after supplementary studies and field experiments.  <em>Tuta absoluta </em>is a serious pest of Solanaceae plants which is resistant to many commonly used insecticides causing economic damage worldwide. Thus, the development of integrated pest management (IPM) approaches against this pest is necessary. This study evaluated the toxicity of matrine (Rui agro®) insecticide against different biological stages of <em>T. absoluta</em>. Analysis revealed egg as the most resistant biological stage with LC₅₀ value 1064.32 mg/L. However, the sublethal concentration (LC₂₅) effect of matrine on <em>T. absoluta</em> population growth parameters was estimated using age-stage, two-sex life table theory method. The sublethal concentration (LC₂₅= 838.25 mg/L) of matrine reduced different biological stages longevity and female fecundity in comparison to control treatment (distilled water). Values of population growth parameters: the intrinsic rate of increase (<em>r</em>), net reproductive rate (<em>R</em>₀), finite rate of increase (<em>λ</em>) and gross reproductive rate (<em>GRR</em>) with LC₂₅ of matrine were significantly lower than the control. The selectivity ratio (SR) value indicated that matrine are selective and safe for predator <em>Macrolophus pygmaeus</em>. Based on the results obtained in this study, combined use of <em>M. pygmaeus</em> with matrine can be recommended in <em>T. absoluta</em> integrated management programs, after supplementary studies and field experiments.  https://arpp.tabrizu.ac.ir/article_19084_fda94190fef4f17d737f53937ba7bb58.pdf